Deleted
Sample Discontinued
Experience: None
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Composite

Part:BBa_J52031:Design

Designed by: Jelka Pohar   Group: iGEM06_Slovenia   (2006-10-15)

NF-kappaB dependent promoter + dnMyD88-linker-GFP-linker-PEST191


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 689
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 776
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2014


Design Notes

Promoter has a NotI restriction site. We did not alter DNA sequence, because this site did not affect our part construction and function of promoter. Has mutations and may not work. This part is cloned in plasmid with terminator BBa_J52017.




Source

NFkB - dnMyD88-linker-GFP-linker-PEST191 - already described (see BBa_J52030)


References